Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.

Applications:

ELISA, IHC

Name of antibody:

PSMD8

Immunogen:

Fusion protein of human PSMD8

Full name:

proteasome (prosome, macropain) 26S subunit, non-ATPase, 8

Synonyms：

S14, p31, HIP6, HYPF, Nin1p, Rpn12

SwissProt:

P48556

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human colon cancer and human lung cancer

IHC Recommend dilution:

50-200