Background:

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

Applications:

WB

Name of antibody:

mGFP

Immunogen:

Purified mGFP protein expressed in HEK293 cell

Full name:

mGFP

SwissProt:

P42212

WB Predicted band size:

27 kDa

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCFP ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

500-2000