Background:

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

Applications:

WB

Name of antibody:

eGFP

Immunogen:

eGFP tagged recombinant protein expressed in HEK293T cell

SwissProt:

P42212

WB Predicted band size:

27 kDa

WB Positive control:

eGFP-fused protein level by using anti-eGFP antibody in HeLa cells after 24 h (Day 1) or 45 h (Day 2); HA-β-globin, co-transfection control EGFP and tubulin (loading control) are indicated.

WB Recommended dilution:

1000-5000